2WAC
Extended Tudor domain of Drosophila Melanogaster Tudor-SN (p100)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Collection date | 2007-06-02 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.560, 47.040, 129.770 |
| Unit cell angles | 90.00, 107.32, 90.00 |
Refinement procedure
| Resolution | 19.640 - 2.100 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hqe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.037 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.640 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.150 |
| Number of reflections | 28296 | |
| <I/σ(I)> | 24.4 | 10.2 |
| Completeness [%] | 98.7 | 97.8 |
| Redundancy | 3.63 | 3.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 20% PEG 3350, 100 MM BIS-TRIS PH 5.5, 200 MM NACL |
