2W9R
Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.080, 28.230, 38.910 |
| Unit cell angles | 97.44, 106.45, 92.39 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.25400 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.040 |
| Number of reflections | 11540 |
| <I/σ(I)> | 12.4 |
| Completeness [%] | 91.1 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
