2W95
STructure of the Discoidin I from Dictyostelium discoideum in complex with GalNAc at 1.75 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-06 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.404, 274.047, 86.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 1.750 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w94 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.545 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 86561 | |
| <I/σ(I)> | 8.1 | 1.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.5 M LISO4, 100 MM NA HEPES PH 7.5, SUGAR SOAKED IN THE CRYSTAL |
