2W91
Structure of a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.060, 58.850, 96.670 |
| Unit cell angles | 90.00, 110.37, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.129 |
| Rwork | 0.127 |
| R-free | 0.16200 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.590 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.080 | 0.410 |
| Number of reflections | 129975 | |
| <I/σ(I)> | 18.5 | 2.9 |
| Completeness [%] | 97.9 | 86.2 |
| Redundancy | 6.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
