2W8U

SPT with PLP, N100Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Spacegroup name	C 2 2 21
Unit cell lengths	71.720, 109.700, 91.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.420 - 1.500
R-factor	0.157
Rwork	0.156
R-free	0.18700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	1.540
High resolution limit [Å]	1.500	1.500
Rmerge	0.040	0.410
Number of reflections	54786
<I/σ(I)>	22	3.4
Completeness [%]	99.7	98.3
Redundancy	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1