2W8J
SPT with PLP-ser
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.248, 108.375, 91.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.900 - 1.500 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 55582 | |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 99.0 | 89 |
| Redundancy | 8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
