2W8D
Distinct and essential morphogenic functions for wall- and lipo- teichoic acids in Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.627, 54.408, 140.783 |
| Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
| Resolution | 141.420 - 2.350 |
| R-factor | 0.174 |
| Rwork | 0.170 |
| R-free | 0.23500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.520 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.360 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.120 | 0.360 |
| Number of reflections | 704196 | |
| <I/σ(I)> | 22.1 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 150 MM MGCL2, 100 MM BICINE, PH 8.0 AND 25 % (W/V) PEG 1500 |
