2W87
Xyl-CBM35 in complex with glucuronic acid containing disaccharide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.850, 45.450, 48.910 |
| Unit cell angles | 71.79, 89.74, 81.28 |
Refinement procedure
| Resolution | 24.150 - 1.600 |
| R-factor | 0.145 |
| Rwork | 0.143 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w46 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.371 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.110 | 0.220 |
| Number of reflections | 30820 | |
| <I/σ(I)> | 11.7 | 3.4 |
| Completeness [%] | 91.9 | 80.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 20% PEG 3350, 0.4M THIOCYANATE PH 6.9 |
