2W7Z
Structure of the pentapeptide repeat protein EfsQnr, a DNA gyrase inhibitor. Free amines modified by cyclic pentylation with glutaraldehyde.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Collection date | 2008-01-02 |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.930, 63.139, 94.619 |
| Unit cell angles | 90.00, 96.09, 90.00 |
Refinement procedure
| Resolution | 94.070 - 1.600 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22200 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.404 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.600 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.250 |
| Number of reflections | 53909 | |
| <I/σ(I)> | 19.3 | 3.1 |
| Completeness [%] | 94.7 | 86 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PROTEIN - 10 MG/ML, 400 MM AMMONIUM SULFATE, 15 MM HEPES PH 7.5, 1 MM DTT, 1 MM ETDA PRECIPITANT - 100 MM MES PH 6.5, 5-15% PEG 6000, 1M LICL |
