2W7Y
Structure of a Streptococcus pneumoniae solute-binding protein in complex with the blood group A-trisaccharide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.990, 104.900, 97.700 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 97.590 - 2.350 |
| R-factor | 0.21592 |
| Rwork | 0.213 |
| R-free | 0.26311 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.212 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.100 | 0.390 |
| Number of reflections | 31126 | |
| <I/σ(I)> | 22.5 | 5.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
