2W7W
The crystal structure of iron superoxide dismutase from Aliivibrio salmonicida.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.720, 70.720, 170.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1isa |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | 0.340 |
| Number of reflections | 55155 | |
| <I/σ(I)> | 9.5 | 3.6 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 5.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 1 MICROLITER DROPS OF A 6.5 MG/ML PROTEIN SOLUTION WITH A SOLUTION CONTAINING 0.1M TRIS-HCL, 1.4 M SODIUM CITRATE, PH 8.5 AT 291K. |
