2W7U

SplA serine protease of Staphylococcus aureus (2.4A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	294
Detector technology	IMAGE PLATE
Collection date	2007-02-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	73.994, 82.216, 134.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 2.430
R-factor	0.211
Rwork	0.209
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2as9
RMSD bond length	0.006
RMSD bond angle	0.632
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.560
High resolution limit [Å]	2.430	2.430
Rmerge	0.080	0.360
Number of reflections	30882
<I/σ(I)>	16.8	4.1
Completeness [%]	98.2	97.2
Redundancy	4.7	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2M SODIUM FORMATE, 20% PEG 3350