2W7T
Trypanosoma brucei CTPS - glutaminase domain with bound acivicin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-11 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.480, 63.680, 76.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.780 - 2.100 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vcn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.220 |
| Number of reflections | 14866 | |
| <I/σ(I)> | 13.9 | 6.16 |
| Completeness [%] | 99.1 | 93.4 |
| Redundancy | 7.84 | 5.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.15 M POTASSIUM BROMIDE, 30 % PEG MONOMETHYL ETHER 2000 |
