2W7R
Structure of the PDZ domain of Human Microtubule associated serine- threonine kinase 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2008-06-21 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 100.940, 100.940, 69.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.190 - 1.600 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.833 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.140 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.050 | |
| Number of reflections | 32248 | |
| <I/σ(I)> | 7.8 | 1.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 75MM NA PHOSPHATE, 91MM K PHOSPHATE |
