2W7Q
Structure of Pseudomonas aeruginosa LolA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.656, 68.439, 61.350 |
| Unit cell angles | 90.00, 94.83, 90.00 |
Refinement procedure
| Resolution | 45.600 - 1.880 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ua8 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.045 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.280 |
| Number of reflections | 27714 | |
| <I/σ(I)> | 20.9 | 4.9 |
| Completeness [%] | 98.9 | 98.8 |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5 30% PEG4000 0.2 M LI2SO4 |
