2W76

Structures of P. aeruginosa FpvA bound to heterologous pyoverdines: FpvA-Pvd(Pa6)-Fe complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	193.422, 129.932, 140.877
Unit cell angles	90.00, 130.77, 90.00

Refinement procedure

Resolution	97.130 - 2.800
R-factor	0.217
Rwork	0.215
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o5p
RMSD bond length	0.019
RMSD bond angle	1.985
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.090	0.370
Number of reflections	59938
<I/σ(I)>	7.9	2.7
Completeness [%]	92.5	83.5
Redundancy	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1