2W6X
Crystal structure of Sperm Whale Myoglobin mutant YQRF in complex with Xenon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.781, 90.781, 45.279 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.750 - 1.730 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1naz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.165 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 1.950 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.080 | 0.030 |
| Number of reflections | 21861 | |
| <I/σ(I)> | 15.4 | 6.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 8.4 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 2.8M AMMONIUM SULFATE, 0.1M TRIS/HCL, PH8.7, HANGING DROP AT RT |
