2W6I
Low resolution structures of bovine mitochondrial F1-ATPase during controlled dehydration: Hydration State 4B.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2008-10-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.920, 131.330, 267.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 4.000 |
| R-factor | 0.299 |
| Rwork | 0.299 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bmf |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0038) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.000 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.220 | 0.600 |
| Number of reflections | 30825 | |
| <I/σ(I)> | 14.3 | 1.7 |
| Completeness [%] | 93.3 | 94.5 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 50 MM TRIS-HCL PH 8.2, 200 MM NACL, 20 MM MGSO4, 1 MM ADP, 1 MM ALCL3, 6 MM NAF 0.004% (W/V)PHENYLMETHYLSULFONYL FLUORIDE AND 12% (W/V) POLYETHYLENE GLYCOL 6000 |
