2W6A
X-ray Structure of the Dimeric GIT1 Coiled-Coil Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.927, 28.962, 46.262 |
| Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
| Resolution | 19.400 - 1.400 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.305 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.460 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.070 | 0.200 |
| Number of reflections | 22529 | |
| <I/σ(I)> | 23.5 | 7.8 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS-HCL PH 8.5, 31% PEG 4000, 0.8M NACL |
