2W69
Influenza polymerase fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.130, 67.130, 302.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.810 - 2.050 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.363 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.060 | 0.690 |
| Number of reflections | 41831 | |
| <I/σ(I)> | 17.6 | 2.2 |
| Completeness [%] | 93.2 | 99.4 |
| Redundancy | 4.84 | 5.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN SOLUTION WAS AT 5-10 MG.ML-1 IN 20 MM TRIS-HCL PH 8.0, 100 MM NACL, 2.5 MM MNCL2. THE RESERVOIR COMPOSITION WAS 100 MM MES PH 6.0, 1.2 M LI2SO4, 10 MM MG ACETATE, 3 % ETHYLENE GLYCOL. |
