2W68

ENHANCING THE RECEPTOR AFFINITY OF THE SIALIC ACID-BINDING DOMAIN OF VIBRIO CHOLERAE SIALIDASE THROUGH MULTIVALENCY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 2 2 21
Unit cell lengths	138.600, 197.620, 82.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.880 - 2.500
R-factor	0.217
Rwork	0.215
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.020
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.570
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.410
Number of reflections	37806
<I/σ(I)>	15.8	2.7
Completeness [%]	95.0	89
Redundancy	4.9	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1