2W65
Anti citrullinated Collagen type 2 antibody acc4 in complex with a citrullinated peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.200, 127.800, 71.900 |
| Unit cell angles | 90.00, 106.00, 90.00 |
Refinement procedure
| Resolution | 24.230 - 2.210 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w60 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.840 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.400 | 2.310 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 45714 | |
| <I/σ(I)> | 14.6 | 4.4 |
| Completeness [%] | 96.6 | 97.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.15 M AMMONIUM SULFATE, 20% PEG 3350 AND 10% GLYCEROL |
