2W60

Anti citrullinated Collagen type 2 antibody acc4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	44.800, 70.000, 136.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.300 - 1.500
R-factor	0.171
Rwork	0.169
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vl5
RMSD bond length	0.017
RMSD bond angle	1.462
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0044)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.200	1.550
High resolution limit [Å]	1.450	1.450
Rmerge	0.070	0.280
Number of reflections	64156
<I/σ(I)>	20.5	6.3
Completeness [%]	97.4	97.7
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		0.15 M AMMONIUM NITRATE, 25% PEG 3350, pH 8