2W5S
Structure-based mechanism of lipoteichoic acid synthesis by Staphylococcus aureus LtaS.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.992, 57.124, 160.357 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.840 - 2.100 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w5q |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.504 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 53.840 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.070 |
| Number of reflections | 24636 |
| <I/σ(I)> | 6.3 |
| Completeness [%] | 99.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 30% PEG4000, 100MM SODIUM CITRATE, PH5.6, 200MM AMMONIUM ACETATE, 30MG/ML GLYCEROL-PHOSPHATE |
