2W5Q
Structure-based mechanism of lipoteichoic acid synthesis by Staphylococcus aureus LtaS.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98, 0.9794, 0.9797, 0.9717 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.214, 57.123, 159.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.030 - 1.200 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.18500 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.143 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.030 |
| High resolution limit [Å] | 1.200 |
| Rmerge | 0.060 |
| Number of reflections | 119440 |
| <I/σ(I)> | 5.7 |
| Completeness [%] | 94.1 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 30% PEG4000, 100MM SODIUM CITRATE, PH5.6, 200MM AMMONIUM ACETATE |
