2W5O
Complex structure of the GH93 alpha-L-arabinofuranosidase of Fusarium graminearum with arabinobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.620, 86.880, 108.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.930 - 2.050 |
| R-factor | 0.171 |
| Rwork | 0.168 |
| R-free | 0.21600 |
| Structure solution method | SAD |
| Starting model (for MR) | 2w5n |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.584 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.620 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.130 | 0.430 |
| Number of reflections | 29532 | |
| <I/σ(I)> | 8.3 | 2.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 22% PEG 4K, 100 MM HEPES 7.5, 50 MM AMSO4, 20% GLYCEROL FOR CRYOPROTECTION |
