2W5N
Native structure of the GH93 alpha-L-arabinofuranosidase of Fusarium graminearum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.340, 90.490, 92.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.270 - 1.850 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FIRST MODEL FROM SELENOMET DATA |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.502 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.270 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 35097 | |
| <I/σ(I)> | 12.3 | 2.9 |
| Completeness [%] | 99.2 | 96.3 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 10% PEG8K 100MM TRIS PH 8.5 100MM MGCL2 | ||
| 1 | 8.5 | 10% PEG8K 100MM TRIS PH 8.5 100MM MGCL2 |
