2W5K
RNASE A-NADPH COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-17 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.962, 33.723, 74.145 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 74.120 - 1.700 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.375 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.030 | 0.120 |
| Number of reflections | 27237 | |
| <I/σ(I)> | 26.6 | 8.1 |
| Completeness [%] | 95.6 | 92.3 |
| Redundancy | 3.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 20% PEG 4000, 0.02M SODIUM CITRATE BUFFER, PH 5.5 |
