2W5F
High resolution crystallographic structure of the Clostridium thermocellum N-terminal endo-1,4-beta-D-xylanase 10B (Xyn10B) CBM22-1- GH10 modules complexed with xylohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-28 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 173.178, 173.178, 131.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 150.760 - 1.900 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.19400 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.318 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.800 | 2.110 |
| High resolution limit [Å] | 2.000 | 1.900 |
| Rmerge | 0.120 | 1.000 |
| Number of reflections | 176859 | |
| <I/σ(I)> | 17.3 | 1.7 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 19.2 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 292 | 1 M NA ACETATE, 0.1 M HEPES PH 7.5, 0.05 M CDSO4, 10 MM XYLOHEXAOSE AT 292K |
