2W50
N-terminal domain of human conserved dopamine neurotrophic factor (CDNF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.300, 42.040, 51.990 |
| Unit cell angles | 90.00, 97.67, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.283 |
| Data scaling software | XDS |
| Phasing software | HKL2MAP |
| Refinement software | REFMAC (5.5.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 110661 | |
| <I/σ(I)> | 13.6 | 3.2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | CRYSTALS OF CDNF AT 10-20 MG/ML WERE GROWN IN SITTING DROPS OVER A RESERVOIR SOLUTION OF 100 MM NA-ACETATE, PH 4.6, 0.2 M NH4-ACETATE AND 25-30 % MME-PEG 2000. |
