2W4Z
Caulobacter bacteriophage 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-25 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 273.600, 273.600, 640.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.296 |
| Rwork | 0.296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CB5-VLP MODEL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.448 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.760 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.180 | 0.650 |
| Number of reflections | 163899 | |
| <I/σ(I)> | 3.6 | 0.9 |
| Completeness [%] | 79.0 | 48 |
| Redundancy | 1.7 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS-HCL PH 8.0, 5% PEG 3350 |
