2W4Y
Caulobacter bacteriophage 5 - virus-like particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 406.400, 342.600, 409.700 |
| Unit cell angles | 90.00, 93.19, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.278 |
| Rwork | 0.278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.130 | 0.950 |
| Number of reflections | 720797 | |
| <I/σ(I)> | 3.9 | 0.7 |
| Completeness [%] | 59.0 | 39 |
| Redundancy | 1.5 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS-HCL PH 8.0, 5% PEG 3350 |
