2W4P
Human common-type acylphosphatase variant, A99G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-04 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.206, 57.339, 58.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.030 - 1.700 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN COMMON-TYPE ACYPHOSPHATASE WILD TYPE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.550 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.100 | 0.240 |
| Number of reflections | 9498 | |
| <I/σ(I)> | 8.5 | 2.1 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 6.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.4M SODIUM MALONATE, PH7.0 25% GLYCEROL |
