2W4J

X-ray structure of a DAP-Kinase 2-277

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	46.844, 62.336, 88.345
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.390 - 1.300
R-factor	0.132
Rwork	0.131
R-free	0.15900
Structure solution method	MAD
Starting model (for MR)	1jks
RMSD bond length	0.020
RMSD bond angle	1.874
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0044)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.260	1.370
High resolution limit [Å]	1.300	1.300
Rmerge	0.080	0.560
Number of reflections	64311
<I/σ(I)>	17	2.7
Completeness [%]	100.0	100
Redundancy	6.5	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1