2W4F
CRYSTAL STRUCTURE OF THE FIRST PDZ DOMAIN OF HUMAN SCRIB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.216, 41.829, 49.897 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.940 - 1.300 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x5q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.380 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0055) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.810 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 71804 | |
| <I/σ(I)> | 14.5 | 4.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 0.15 M AMMONIUM CL PH 6.3, 20 % PEG 1000, 15% ETHYLENE GLYCOL |
