2W48

Crystal structure of the Full-length Sorbitol Operon Regulator SorC from Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	91.670, 113.330, 184.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.672 - 3.200
R-factor	0.2194
Rwork	0.218
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gnp
RMSD bond length	0.014
RMSD bond angle	1.477
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.150	0.680
Number of reflections	32577
<I/σ(I)>	7.6	2
Completeness [%]	99.9	100
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1