2W47
Clostridium thermocellum CBM35 in complex with delta-4,5- anhydrogalacturonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 86.033, 86.033, 75.905 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.540 - 1.400 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.190 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.540 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.080 | 0.640 |
| Number of reflections | 31544 | |
| <I/σ(I)> | 24.1 | 3.7 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 13 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.6M SODIUM CITRATE |
