2W43
Structure of L-haloacid dehalogenase from S. tokodaii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-21 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.796, 71.889, 85.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.050 - 1.660 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qq5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.329 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.050 | 0.230 |
| Number of reflections | 51594 | |
| <I/σ(I)> | 28.8 | 7.3 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M MES, 20% PEG6000, PH6 |
