2W42
THE STRUCTURE OF A PIWI PROTEIN FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH A 16NT DNA DUPLEX.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.838, 61.384, 103.540 |
| Unit cell angles | 75.80, 75.86, 79.47 |
Refinement procedure
| Resolution | 98.060 - 1.900 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w9h |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.766 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005 24/04/2001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.720 | 2.000 |
| High resolution limit [Å] | 1.800 | 1.900 |
| Rmerge | 0.050 | 0.420 |
| Number of reflections | 89928 | |
| <I/σ(I)> | 9.62 | 1.6 |
| Completeness [%] | 95.9 | 95.5 |
| Redundancy | 2.09 | 2.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | pH 4.6 |
