2W3T
Chloro complex of the Ni-Form of E.coli deformylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-04-08 |
| Detector | RIGAKU CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.080, 35.980, 67.590 |
| Unit cell angles | 90.00, 113.43, 90.00 |
Refinement procedure
| Resolution | 62.020 - 1.690 |
| R-factor | 0.21594 |
| Rwork | 0.213 |
| R-free | 0.24322 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a18 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.078 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.050 | 0.280 |
| Number of reflections | 19326 | |
| <I/σ(I)> | 18 | 1.8 |
| Completeness [%] | 94.2 | 56.5 |
| Redundancy | 4.29 | 1.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 293 | 20.5% PEG4000, 100MM NAOAC PH 4.0, 293 K |
