2W3C
Globular head region of the human general vesicular transport factor p115
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.550, 68.890, 85.750 |
| Unit cell angles | 90.00, 108.74, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.220 |
| R-factor | 0.222 |
| Rwork | 0.219 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.450 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 2.350 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.070 | |
| Number of reflections | 43877 | |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 90.5 | 82.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
