2W39
Glc(beta-1-3)Glc disaccharide in -1 and -2 sites of Laminarinase 16A from Phanerochaete chrysosporium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.147, 46.713, 152.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 1.100 |
| R-factor | 0.138 |
| Rwork | 0.138 |
| R-free | 0.15200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cl2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.450 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.550 | 1.110 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 98347 | |
| <I/σ(I)> | 15.43 | 2.3 |
| Completeness [%] | 88.3 | 50.2 |
| Redundancy | 3.84 | 2.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 10 MG/ML LAMINARINASE 16A IN 25 % PEG 3350, 0.2 M AMMONIUM NITRATE AND 10 MM NAOAC PH 5, CO-CRYSTALIZED WITH 10 MM 4-O-BETA-D-GLUCOSYL-LAMINARIBIOSE (G4G3G) LIGAND FOR 6 MONTHS |
