2W2N
WT PCSK9-deltaC bound to EGF-A H306Y mutant of LDLR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-08 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 83.940, 83.940, 209.933 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.300 |
R-factor | 0.21531 |
Rwork | 0.213 |
R-free | 0.25393 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qtw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.188 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.150 | 0.640 |
Number of reflections | 33566 | |
<I/σ(I)> | 11.7 | 2.9 |
Completeness [%] | 98.1 | 99.7 |
Redundancy | 7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1M HEPES PH 7.5, 10% (W/V) PEG 8000, 8% (V/V) ETHYLENE GLYCOL. |