2W21
Crystal structure of the aminoacid kinase domain of the glutamate 5 kinase of Escherichia coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-26 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 141.358, 141.358, 78.373 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.950 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j5t |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.901 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 122.170 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.110 | 0.440 |
| Number of reflections | 10173 | |
| <I/σ(I)> | 5.6 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.4 | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | LITHIUM SULFATE 1M, AMMONIUM SULFATE 0.5M, SODIUM SITRATE 0.1M PH 5.6 |
