2W1N
cohesin and fibronectin type-III double module construct from the Clostridium perfringens glycoside hydrolase GH84C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.245, 48.644, 36.900 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 73.720 - 1.800 |
| R-factor | 0.228 |
| Rwork | 0.224 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o4e |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.732 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.720 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.500 |
| Number of reflections | 27684 | |
| <I/σ(I)> | 30.2 | 1.9 |
| Completeness [%] | 94.0 | 94.5 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.5 | pH 3.5 |
