2W1K
Crystal Structure of Sortase C-3 (SRTC-3) from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ROTAFLEX |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-31 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 122.638, 122.638, 122.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.350 - 2.140 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w1j |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.296 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.350 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 31007 | |
| <I/σ(I)> | 14.3 | 4.2 |
| Completeness [%] | 90.8 | 76.3 |
| Redundancy | 6.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 100 MM MES PH 5.0, 0.5 M (NH4)2SO4, 1.0 M LISO4 |
