2W16
Structures of FpvA bound to heterologous pyoverdines
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.220, 130.082, 141.783 |
| Unit cell angles | 90.00, 130.67, 90.00 |
Refinement procedure
| Resolution | 107.830 - 2.710 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o5p |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.831 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.4.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 70306 | |
| <I/σ(I)> | 9 | 2.7 |
| Completeness [%] | 95.4 | 95.2 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.3M NAH2PO4, 0.1M MES, PH 6.5 |
