2W10
Mona SH3C in complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.137, 34.367, 36.220 |
| Unit cell angles | 81.08, 87.05, 72.55 |
Refinement procedure
| Resolution | 35.780 - 1.900 |
| R-factor | 0.164 |
| Rwork | 0.161 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uti |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.644 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.040 | 0.060 |
| Number of reflections | 8966 | |
| <I/σ(I)> | 18.7 | 11.5 |
| Completeness [%] | 81.9 | 44.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M PHOSPHATE CITRATE, 2 M AMMONIUM SULPHATE, pH 7.5 |
