2W0O
Horse spleen apoferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-20 |
| Detector | ADSC CCD |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.000, 182.000, 182.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.830 - 1.500 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v2i |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.556 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.750 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.550 |
| Number of reflections | 77238 | |
| <I/σ(I)> | 32.2 | 4.6 |
| Completeness [%] | 98.9 | 98.6 |
| Redundancy | 11.1 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | RESERVOIR: CADMIUM SULFATE 0.12M, AMMONIUM SULFATE 0.8M, SODIUM ACETATE BUFFER 0.1M PH 5.6, SODIUM AZIDE 0.003M. DROP: 1UL PROTEIN AND 1 UL RESERVOIR |
