2W01
Crystal structure of the guanylyl cyclase Cya2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.445, 84.090, 115.328 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.310 |
| R-factor | 0.198 |
| Rwork | 0.194 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wc1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.922 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 50776 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 97.3 | 85 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
